Amflora

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Amflora
Auspflanzung Schweden 2.jpg
EventEH92-527-1
Identifier(s)BPS-25271-9
Plant Solanum tuberosum L.
Mode Transgenesis
Method Insertion
Vector pHoxwG [1]
Developer Svalöf Weibull AB [1]
Trait(s) conferred Decreased amylose production, increased kanamycin resistance
Genes introduced Granule bound starch synthase, neomycin phosphotransferase II

Amflora (also known as EH92-527-1) is a genetically modified potato cultivar developed by BASF Plant Science. "Amflora" potato plants produce pure amylopectin starch that is processed to waxy potato starch. It was approved for industrial applications in the European Union on 2 March 2010 by the European Commission. [2] In January 2012, the potato was withdrawn from the market in the EU.

Contents

History

Originally registered on 5 August 1996, Amflora was developed by geneticist Lennart Erjefält and agronomist Jüri Känno of Svalöf Weibull AB. [1]

After the European Commission's approval of the potato, BASF announced it was going to produce Amflora seed starting in April 2010 in Germany's Western Pomerania (20 ha) and Sweden (80 ha). It also announced it was planting 150 ha in the Czech Republic "for commercial aims with an unnamed partner." [3]

Due to lack of acceptance of GM crops in Europe, BASF Plant Science decided in January 2012 to stop its commercialization activities in Europe and would no longer sell Amflora there, but it would continue seeking regulatory approval for its products in the Americas and Asia. [4]

In 2013, an EU court annulled the approval of BASF's Amflora, saying that the EU Commission broke rules when it approved the potato in 2010. [5]

Biology

Waxy potato varieties produce two main kinds of potato starch, amylose and amylopectin, the latter of which is most industrially useful. The Amflora potato has been modified to contain antisense RNA against the enzyme that drives synthesis of amylose, namely granule bound starch synthase. [6] This resulting potato almost exclusively produces amylopectin, and thus is more useful for the starch industry.

Industrial applications

Regular potato starch contains two constituent types of molecules: amylopectin (80 percent), which is more useful as a polymer for industry, and amylose (20 percent) which often creates problems as starch retrogradation, so must be modified with chemical reactions which can be costly. [7]

After two decades of research efforts, [8] BASF's biotechnologists using genetic engineering succeeded in creating a potato, named "Amflora", where the gene responsible for the synthesis of amylose had been turned off, thus the potato is unable to synthesize the less desirable amylose.

Amflora potatoes would be processed and sold as starch to industries that prefer waxy potato starch with only amylopectin. Amflora is intended only for industrial applications such as papermaking and other technical applications. [9] Europe produces more than two million metric tons of natural potato starch a year, and BASF with its Amflora product hoped to enter into this large market. [7]

Other possible uses

According to The New York Times , BASF has a second application pending for use of Amflora's potato pulp as animal feed. [8]

Political disagreements

Protests against the Amflora potato BurGenLand (2010).jpg
Protests against the Amflora potato

Various environmental organizations, such as Greenpeace, disagreed with the introduction of the Amflora genetically modified potato into the market. The lengthy approval process frustrated some supporters of the potato. A BASF scientist said to The New York Times, "it's hard when you see an innovative product go through the loops again and again. These decisions are not about science but about politics". [8] After the potato was approved, the European Greens political party and the then Italian agricultural minister Luca Zaia criticized the approval. [10] The International Peasant Movement La Via Campesina issued a press release on 8 March 2010 also criticizing the decision. [11]

Reactions by Greek politicians

After Amflora's licensing by the European Commission on 2 March 2010, the Coalition of the Radical Left's Member of Parliament for the A Thessalonikis prefecture, Tasos Kouvelis, asked the Greek Minister of Agriculture on 3 March 2010 to declare the production of the potato illegal in Greece. [12] On 4 March 2010 Panhellenic Socialist Movement's European Member of Parliament Kriton Arsenis submitted a question at Europarl asking about the consequences of Amflora. [13]

PASOK's MP Maria Damanaki accepted the decision of the European Commission, while Greek Agriculture Minister Katerina Batzeli said the production of Amflora will not be allowed in Greece. [14]

Licensing procedure

Amflora could not be sold within the European Union without approval, and its licence could only be issued after voting at the Council of Ministers of the European Union with a 74 percent threshold of support. Two rounds of voting were held, first by experts in December 2006 and then by the agricultural ministers in July 2007, but both failed to reach the 74 percent threshold. Although the voting was by secret ballot, The New York Times reported that Amflora was supported by the agricultural ministers of Germany and Belgium, and was opposed by the agricultural ministers of Italy, Ireland, and Austria, while the agricultural ministers of France and Bulgaria abstained from voting. [8]

After a licence was issued on 2 March 2010, BASF announced its intention to ask for approval of more varieties of genetically modified potatoes, such as the "Fortuna" potato. [3]

Related Research Articles

<span class="mw-page-title-main">Biotechnology</span> Use of living systems and organisms to develop or make useful products

Biotechnology is the integration of natural sciences and engineering sciences in order to achieve the application of organisms, cells, parts thereof and molecular analogues for products and services. The term biotechnology was first used by Károly Ereky in 1919, meaning the production of products from raw materials with the aid of living organisms.

<span class="mw-page-title-main">Potato</span> Staple food, root tuber, starchy

The potato is a starchy food, a tuber of the plant Solanum tuberosum and is a root vegetable native to the Americas. The plant is a perennial in the nightshade family Solanaceae.

<span class="mw-page-title-main">Polysaccharide</span> Long carbohydrate polymers comprising starch, glycogen, cellulose, and chitin

Polysaccharides, or polycarbohydrates, are the most abundant carbohydrates found in food. They are long-chain polymeric carbohydrates composed of monosaccharide units bound together by glycosidic linkages. This carbohydrate can react with water (hydrolysis) using amylase enzymes as catalyst, which produces constituent sugars. They range in structure from linear to highly branched. Examples include storage polysaccharides such as starch, glycogen and galactogen and structural polysaccharides such as cellulose and chitin.

<span class="mw-page-title-main">Starch</span> Glucose polymer used as energy store in plants

Starch or amylum is a polymeric carbohydrate consisting of numerous glucose units joined by α-(1→4)-D glycosidic bonds. This polysaccharide is produced by most green plants for energy storage. Worldwide, it is the most common carbohydrate in human diets, and is contained in large amounts in staple foods such as wheat, potatoes, maize (corn), rice, and cassava (manioc).

<span class="mw-page-title-main">Amylase</span> Class of enzymes

An amylase is an enzyme that catalyses the hydrolysis of starch into sugars. Amylase is present in the saliva of humans and some other mammals, where it begins the chemical process of digestion. Foods that contain large amounts of starch but little sugar, such as rice and potatoes, may acquire a slightly sweet taste as they are chewed because amylase degrades some of their starch into sugar. The pancreas and salivary gland make amylase to hydrolyse dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. Plants and some bacteria also produce amylase. Specific amylase proteins are designated by different Greek letters. All amylases are glycoside hydrolases and act on α-1,4-glycosidic bonds.

<span class="mw-page-title-main">Mochi</span> Japanese rice cake

Mochi is a Japanese rice cake made of mochigome (もち米), a short-grain japonica glutinous rice, and sometimes other ingredients such as water, sugar, and cornstarch. The rice is pounded into paste and molded into the desired shape. In Japan, it is traditionally made in a ceremony called mochitsuki. While eaten year-round, mochi is a traditional food for the Japanese New Year, and is commonly sold and eaten during that time.

<span class="mw-page-title-main">BASF</span> German chemicals company

BASF SE is a European multinational chemical company and the largest chemical producer in the world. Its headquarters are located in Ludwigshafen, Germany.

<span class="mw-page-title-main">Genetically modified food</span> Foods produced from organisms that have had changes introduced into their DNA

Genetically modified foods, also known as genetically engineered foods, or bioengineered foods are foods produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Genetic engineering techniques allow for the introduction of new traits as well as greater control over traits when compared to previous methods, such as selective breeding and mutation breeding.

<span class="mw-page-title-main">Dextrin</span> Chemical compound

Dextrins are a group of low-molecular-weight carbohydrates produced by the hydrolysis of starch and glycogen. Dextrins are mixtures of polymers of D-glucose units linked by α-(1→4) or α-(1→6) glycosidic bonds.

<span class="mw-page-title-main">Amylose</span> Chemical compound

Amylose is a polysaccharide made of α-D-glucose units, bonded to each other through α(1→4) glycosidic bonds. It is one of the two components of starch, making up approximately 20–30%. Because of its tightly packed helical structure, amylose is more resistant to digestion than other starch molecules and is therefore an important form of resistant starch.

<span class="mw-page-title-main">Amylopectin</span> Chemical compound

Amylopectin is a water-insoluble polysaccharide and highly branched polymer of α-glucose units found in plants. It is one of the two components of starch, the other being amylose.

Starch gelatinization is a process of breaking down of intermolecular bonds of starch molecules in the presence of water and heat, allowing the hydrogen bonding sites to engage more water. This irreversibly dissolves the starch granule in water. Water does act as a plasticizer.

<span class="mw-page-title-main">Genetically modified crops</span> Plants used in agriculture

Genetically modified crops are plants used in agriculture, the DNA of which has been modified using genetic engineering methods. Plant genomes can be engineered by physical methods or by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors. In most cases, the aim is to introduce a new trait to the plant which does not occur naturally in the species. Examples in food crops include resistance to certain pests, diseases, environmental conditions, reduction of spoilage, resistance to chemical treatments, or improving the nutrient profile of the crop. Examples in non-food crops include production of pharmaceutical agents, biofuels, and other industrially useful goods, as well as for bioremediation.

<span class="mw-page-title-main">Cycloamylose</span>

Cycloamyloses are cyclic α-1,4 linked glucans comprising dozens or hundreds of glucose units. Chemically they are similar to the much smaller cyclodextrins, which are typically composed of 6, 7 or 8 glucose units.

<span class="mw-page-title-main">Resistant starch</span> Dietary fiber

Resistant starch (RS) is starch, including its degradation products, that escapes from digestion in the small intestine of healthy individuals. Resistant starch occurs naturally in foods, but it can also be added as part of dried raw foods, or used as an additive in manufactured foods.

<span class="mw-page-title-main">Waxy corn</span>

Waxy corn or glutinous corn is a type of field corn characterized by its sticky texture when cooked as a result of larger amounts of amylopectin. The corn was first described from a specimen from China in 1909. As this plant showed many peculiar traits, the American breeders long used it as a genetic marker to tag the existence of hidden genes in other maize breeding programs. In 1922 a researcher found that the endosperm of waxy maize contained only amylopectin and no amylose starch molecule in opposition to normal dent corn varieties that contain both. Until World War II, the main source of starch in the United States was tapioca, but when Japan severed the supply lines of the U.S., they forced processors to turn to waxy maize. Amylopectin or waxy starch is now used mainly in food products, but also in the textile, adhesive, corrugating and paper industry.

Waxy potato starch is a variety of commercially available starch composed almost entirely of amylopectin molecules, extracted from new potato varieties. Standard starch extracted from traditional potato varieties contains both amylose and amylopectin.

BASF Plant Science is a subsidiary of BASF in which all plant biotechnology activities are consolidated. The company was founded in 1998 and employs approximately 700 people at 6 different locations worldwide. The headquarters of BASF Plant Science is located in Research Triangle Park and has research sites in the US, Canada, and Europe. The company mainly develops genetically modified seeds at these locations.

Genetic engineering in the European Union has varying degrees of regulation.

<span class="mw-page-title-main">Genetically modified potato</span> Potato that has had its genes modified using genetic engineering

A genetically modified potato is a potato that has had its genes modified, using genetic engineering. Goals of modification include introducing pest resistance, tweaking the amounts of certain chemicals produced by the plant, and to prevent browning or bruising of the tubers. Varieties modified to produce large amounts of starches may be approved for industrial use only, not for food.

References

  1. 1 2 3 "Notification for Placing the Potato Clone EH92-527-1, Being Genetically Modified for Increased Content of Amylopectin, on the Market" (PDF). Archived from the original (PDF) on 2008-11-22. Retrieved 2011-08-17.
  2. "European Commission approves Amflora starch potato - BASF - the Chemical Company - Corporate Website". Archived from the original on 2012-12-06. Retrieved 2010-03-26.
  3. 1 2 "GM potatoes: BASF at work". Archived from the original on 2010-05-31. Retrieved 2010-03-26.
  4. James Kanter for The New York Times. January 16, 2012. BASF to Stop Selling Genetically Modified Products in Europe
  5. Dunmore, Charlie (Dec 13, 2013). "EU court annuls approval of BASF's Amflora GMO potato" . Retrieved 26 February 2015.
  6. "GMO compass database". Archived from the original on 2014-10-09. Retrieved 6 October 2014.
  7. 1 2 http://www.basf.com/group/corporate/en/function/conversions:/publish/content/products-and-industries/biotechnology/plant-biotechnology/images/BASF_Plant_Science_Amflora.pdf [ dead link ]
  8. 1 2 3 4 Rosenthal, Elisabeth (24 July 2007). "A Genetically Modified Potato, Not for Eating, Is Stirring Some Opposition in Europe". The New York Times.
  9. TAGLIABUE, John (June 10, 2010). "A Potato Remade for Industry Has Some Swedes Frowning". The New York Times. Retrieved 26 February 2015.
  10. "ΠΑΣΕΓΕΣ: Η Ευρ. Επιτροπή ενέκρινε την καλλιέργεια της μεταλλαγμένης πατάτας Amflora". Archived from the original on 2011-07-21. Retrieved 2010-03-27.
  11. "La Via Campesina | International Peasants' Movement".
  12. "Να απαγορευτεί η καλλιέργεια της μεταλλαγμένης πατάτας Amflora από τους αγρότες της χώρας μας | Τάσος Κουράκης".
  13. "Ενημέρωση".
  14. "Amflora - 4/3/2010 7:00:00". Archived from the original on 2011-09-27.

Further reading