Blepharisma

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Blepharisma
Mikrofoto.de-Blepharisma japonicum 15.jpg
Blepharisma japonicum
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Clade: Diaphoretickes
Clade: SAR
Clade: Alveolata
Phylum: Ciliophora
Class: Heterotrichea
Order: Heterotrichida
Family: Blepharismidae
Genus: Blepharisma
Perty, 1852

Blepharisma is a genus of unicellular ciliate protists found in fresh and salt water. The group includes about 40 accepted species, and many sub-varieties and strains. While species vary considerably in size and shape, most are easily identified by their red or pinkish color, which is caused by granules of the pigment blepharismin. [1]

Contents

All members of the genus possess a long series of membranelles on the left side of the oral groove, and an "undulating membrane" (a structure resembling a flap, scarf or small sail, composed of long cilia fused together as a single sheet) on the right side of the peristome, toward the posterior. [2]

Certain species of Blepharisma have served as model organisms for scientific research. Since some varieties are easily cultured and readily available from scientific supply houses, they are a common object of study in school science classes.

Appearance

Blepharisma may be as small as 50 micrometres in length, or as large as 1 mm (though normal size range is between 75 and 300 micrometres). [2] Body shape varies within the genus. The type species of the genus, B. persicinum, is ellipsoidal. Blepharisma lateritium is teardrop-shaped, with a rounded posterior; while Blepharisma elongatum and Blepharisma sphagni are long and thin, tapering at the posterior into a tail-like point. [3]

All species are uniformly ciliated, with the cilia arranged in longitudinal rows, and stripes of pigment alternating between rows of cilia. Cilia, short hairlike organelles, sweep food into its mouth and are used for movement. [4] The pink or red pigmentation may be quite pale, and in certain cases it is absent altogether. [5] A contractile vacuole, often quite large, is located in the posterior. The Macronuclei can take a variety of forms. Depending on species and phase of life, they may be rod-shaped, ovoid, spherical, or moniliform (like a rosary, or string of beads).

Reproduction and sexual phenomena

Blepharisma morphology Blepharisma diagram.png
Blepharisma morphology

Like all ciliates, Blepharisma reproduce asexually, by binary fission, dividing transversally. Fission may occur spontaneously, as part of the vegetative cell cycle, or it may follow a sexual phenomenon called conjugation, a process through which genetic material is exchanged between cells. In conjugation, two organisms come into close contact, and a temporary cytoplasmic bridge forms between them. The micronuclei of each cell then undergo meiosis, and haploid micronuclei pass from one individual to the other. This permits the reshuffling of hereditary characteristics, as in other types of sexual reproduction. Conjugation is immediately followed by binary fission of the two conjugants. [6]

In Blepharisma, as in some other ciliates, chemical substances called gamones are used to induce conjugation by stimulating interaction between compatible mating partners. [7]

Although clonal cells of Blepharisma are sometimes able to conjugate with one another (a phenomenon known as selfing), [8] conjugation ordinarily involves the interaction of cells of different mating types. In the species Blepharisma japonicum, there are two mating types (I and II), each type excreting a specific pheromone (termed gamone 1 and gamone 2, respectively). [8] [9] When sexually mature mating-type I cells are moderately starved, they autonomously produce and secrete gamone I. [8] Gamone 1 specifically acts on mating-type II cells, transforming them so that they can unite with type I cells, and inducing them to secrete gamone 2. Gamone 2 then transforms type I cells so that they can unite with type II cells. Cells that can unite may then undergo conjugation. Conjugation of opposite mating types promotes outcrossing and the masking of deleterious recessive mutations in the diploid phase of the sexual cycle. [10]

Feeding and behavior

Blepharisma feed on a variety of smaller organisms, including bacteria, flagellate algae, rotifers, other ciliates and even smaller members of the same species. Experiments with Blepharisma undulans have shown that cannibalism causes gigantism. When individuals are given a diet of smaller Blepharisma, or certain ciliates (particularly Colpidium colpoda or Tetrahymena ), they grow to a relatively enormous size. As long as their diet remains unchanged, cannibal giants will divide to produce more giants. When large prey become unavailable, the offspring will revert to normal size. [11]

Photobiology

Blepharismin C
Blepharismin C.svg
Names
IUPAC name
5,7,11,13,17,19,23,25-octahydroxy-15-(4-hydroxyphenyl)-6,24-di(propan-2-yl)octacyclo[14.11.1.12,10.03,8.04,26.020,28.022,27.014,29]nonacosa-1,3,5,7,10(29),11,13,16,18,20(28),22,24,26-tridecaene-9,21-dione
Identifiers
3D model (JSmol)
ChemSpider
PubChem CID
  • InChI=1S/C41H30O11/c1-11(2)19-36(47)32-30-28-26-22(15(43)9-17(45)24(26)40(51)34(30)38(19)49)21(13-5-7-14(42)8-6-13)23-16(44)10-18(46)25-27(23)29(28)31-33(32)37(48)20(12(3)4)39(50)35(31)41(25)52/h5-12,21,42-50H,1-4H3
    Key: FRDONCXLMWOCKJ-UHFFFAOYSA-N
  • CC(C)C1=C(C2=C3C4=C5C6=C(C(=CC(=C6C(C7=C(C=C(C(=C74)C(=O)C3=C1O)O)O)C8=CC=C(C=C8)O)O)O)C(=O)C9=C(C(=C(C2=C59)O)C(C)C)O)O
Properties
C41H30O11
Molar mass 698.680 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Blepharisma are markedly photophobic, and when light levels are increased will seek out darkened areas. The ability to detect light is accomplished with photosensitive pigment granules located just under the plasma membrane of the cell. The pigment in these granules is blepharismin, the same substance that gives Blepharisma their characteristic pinkish color. [12] Blepharisma are usually pink when collected in nature, but when grown in darkness with abundant food they turn red. Exposure to light or starvation causes them to lose their color, but deeply-pigmented cells can even be killed by strong light. [13]

List of species

Blepharisma hyalinum Blepharisma hyalinum - 160x - Lange 200u (10388446276).jpg
Blepharisma hyalinum
Blepharisma americanum swimming in a drop of pond water, with other microorganisms.
Blepharisma japonicum Mikrofoto.de-Blepharisma japonicum 21.jpg
Blepharisma japonicum

Related Research Articles

<i>Tetrahymena</i> Genus of single-celled organisms

Tetrahymena, a unicellular eukaryote, is a genus of free-living ciliates. The genus Tetrahymena is the most widely studied member of its phylum. It can produce, store and react with different types of hormones. Tetrahymena cells can recognize both related and hostile cells.

<span class="mw-page-title-main">Heterotrich</span> Class of single-celled organisms

The heterotrichs are a class of ciliates. They typically have a prominent adoral zone of membranelles circling the mouth, used in locomotion and feeding, and shorter cilia on the rest of the body. Many species are highly contractile, and are typically compressed or conical in form. These include some of the largest protozoa, such as Stentor and Spirostomum, as well as many brightly pigmented forms, such as certain Blepharisma.

<i>Paramecium</i> Genus of unicellular ciliates, commonly studied as a representative of the ciliate group

Paramecium is a genus of eukaryotic, unicellular ciliates, commonly studied as a model organism of the ciliate group. Paramecium are widespread in freshwater, brackish, and marine environments and are often abundant in stagnant basins and ponds. Because some species are readily cultivated and easily induced to conjugate and divide, they have been widely used in classrooms and laboratories to study biological processes. The usefulness of Paramecium as a model organism has caused one ciliate researcher to characterize it as the "white rat" of the phylum Ciliophora.

<span class="mw-page-title-main">Mating</span> Process of pairing in biology

In biology, mating is the pairing of either opposite-sex or hermaphroditic organisms for the purposes of sexual reproduction. Fertilization is the fusion of two gametes. Copulation is the union of the sex organs of two sexually reproducing animals for insemination and subsequent internal fertilization. Mating may also lead to external fertilization, as seen in amphibians, fishes and plants. For most species, mating is between two individuals of opposite sexes. However, for some hermaphroditic species, copulation is not required because the parent organism is capable of self-fertilization (autogamy); for example, banana slugs.

<i>Stentor</i> (ciliate) Genus of single-celled organisms

Stentor, sometimes called trumpet animalcules, are a genus of filter-feeding, heterotrophic ciliates, representative of the heterotrichs. They are usually horn-shaped, and reach lengths of two millimeters; as such, they are among the largest known extant unicellular organisms. They reproduce asexually through binary fission.

<i>Vorticella</i> Genus of single-celled organisms

Vorticella is a genus of bell-shaped ciliates that have stalks to attach themselves to substrates. The stalks have contractile myonemes, allowing them to pull the cell body against substrates. The formation of the stalk happens after the free-swimming stage.

<i>Didinium</i> Genus of single-celled organisms

Didinium is a genus of unicellular ciliates with at least ten accepted species. All are free-living carnivores. Most are found in fresh and brackish water, but three marine species are known. Their diet consists largely of Paramecium, although they will also attack and consume other ciliates. Some species, such as D. gargantua, also feeds on non-ciliate protists, including dinoflagellates, cryptomonads, and green algae.

<i>Spirostomum</i>

Spirostomum is a genus of ciliated protists in the class Heterotrichea. It is known for being very contractile. Having been first identified by Christian Gottfried Ehrenberg in 1834, further research has identified eight additional true morphospecies. This bacterivore genus mainly lives in the sediment deposits at the bottom of various aquatic habitats, and members possess rquA genes that could be responsible for their ability to survive in these hypoxic and anoxic environments. They are identifiable by their relatively large tubular/flat vermiform bodies. Their life cycle consists of a growth stage, in which they mature, and asexual and sexual reproduction stages. Some species are model organisms for studies on human pathogenic bacteria, while others are sensitive and accurate bioindicators for toxic substances.

<i>Paramecium caudatum</i> Species of single-celled organism

Paramecium caudatum is a species of unicellular protist in the phylum Ciliophora. They can reach 0.33 mm in length and are covered with minute hair-like organelles called cilia. The cilia are used in locomotion and feeding. The species is very common, and widespread in marine, brackish and freshwater environments.

<i>Nassula</i> Genus of single-celled organisms

Nassula is a genus of unicellular ciliates, belonging to the class Nassophorea. Like other members of the class, Nassula possesses a basket-like feeding apparatus made up of cytopharyngeal rods (nematodesmata), which are themselves composed of closely packed microtubules. Nassula use this structure to ingest filamentous cyanobacteria, drawing individual strands of blue-green algae through the cytopharynx and into the body of the cell, where they are digested. As the algae are broken down, they can take on a variety of bright colours, which give Nassula a distinctive, variegated appearance under the microscope.

<span class="mw-page-title-main">Ciliate</span> Taxon of protozoans with hair-like organelles called cilia

The ciliates are a group of alveolates characterized by the presence of hair-like organelles called cilia, which are identical in structure to eukaryotic flagella, but are in general shorter and present in much larger numbers, with a different undulating pattern than flagella. Cilia occur in all members of the group and are variously used in swimming, crawling, attachment, feeding, and sensation.

<i>Chilodonella uncinata</i> Species of single-celled organism

Chilodonella uncinata is a single-celled organism of the ciliate class of alveoles. As a ciliate, C. uncinata has cilia covering its body and a dual nuclear structure, the micronucleus and macronucleus. Unlike some other ciliates, C. uncinata contains millions of minichromosomes in its macronucleus while its micronucleus is estimated to contain 3 chromosomes. Childonella uncinata is the causative agent of Chilodonelloza, a disease that affects the gills and skin of fresh water fish, and may act as a facultative of mosquito larva.

<i>Frontonia</i> Genus of single-celled organisms

Frontonia is a genus of free-living unicellular ciliate protists, belonging to the order Peniculida. As Peniculids, the Frontonia are closely related to members of the genus Paramecium. However, whereas Paramecia are mainly bacterivores, Frontonia are capable of ingesting large prey such as diatoms, filamentous algae, testate amoebas, and even, in some circumstances, members of their own species. In bacteria-rich saprobic conditions, Frontonia leucas can live as a facultative bacterivore.

<i>Climacostomum</i> Genus of single-celled organisms

Climacostomum is a genus of unicellular ciliates, belonging to the class Heterotrichea.

<i>Blepharisma japonicum</i> Species of single-celled organism

Blepharisma japonicum is a species of protozoan that can be found either in water or soil in Japan.

<i>Dileptus margaritifer</i> Species of single-celled organism

Dileptus margaritifer is a species of ciliates in the family Dileptidae. It is common in freshwater streams, lakes and ponds, as well as mosses and soil. The species has been found on every continent except Antarctica.

<span class="mw-page-title-main">Condylostoma</span> Genus of protists belonging to the ciliates phylum

Condylostoma is a genus of unicellular ciliate protists, belonging to the class Heterotrichea.

Parduczia is a genus of karyorelict ciliates in the family Geleiidae.

<i>Halteria</i> Genus of single-celled organisms

Halteria, sometimes referred to as the jumping oligotrich, is a genus of common planktonic ciliates that are found in many freshwater environments. Halteria are easy to locate due to their abundance and distinctive behaviour with observations of Halteria potentially dating back to the 17th century and the discovery of microorganisms. Over time more has been established about their morphology and behavior, which has led to many changes in terms of classification.

Holosticha is a genus of littoral ciliates.

References

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  2. 1 2 Hirshfield; et al. (1965). "A proposed organization of the genus Blepharisma Perty and description of four new species". The Journal of Protozoology. 12 (1): 136–144. doi:10.1111/j.1550-7408.1965.tb01826.x.
  3. Kahl, Alfred (1930–1935). F. Dahl (ed.). Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria) In: Die Tierwelt Deutschlands. Vol. 3 Spirotricha. Jena: G. Fischer. pp. 442–448.
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  12. Matsuoka; et al. (2000). "Analyses of Structure of Photoreceptor Organelle and Blepharismin associated Protein in Unicellular Eukaryote Blepharisma". Photochemistry and Photobiology. 57 (3): 245–255. doi:10.1562/0031-8655(2000)0720709AOSOPO2.0.CO2. PMID   11107859. S2CID   204166047.
  13. Giese, Arthur C. (1981). "The Photobiology of Blepharisma". Photochemical and Photobiological Reviews. Springer, Boston, MA. pp. 139–180. doi:10.1007/978-1-4684-7003-1_4. ISBN   978-1-4684-7005-5.
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Further reading