Nanobiotechnology

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Nanobiotechnology, bionanotechnology, and nanobiology are terms that refer to the intersection of nanotechnology and biology. [1] Given that the subject is one that has only emerged very recently, bionanotechnology and nanobiotechnology serve as blanket terms for various related technologies.

Contents

This discipline helps to indicate the merger of biological research with various fields of nanotechnology. Concepts that are enhanced through nanobiology include: nanodevices (such as biological machines), nanoparticles, and nanoscale phenomena that occurs within the discipline of nanotechnology. This technical approach to biology allows scientists to imagine and create systems that can be used for biological research. Biologically inspired nanotechnology uses biological systems as the inspirations for technologies not yet created. [2] However, as with nanotechnology and biotechnology, bionanotechnology does have many potential ethical issues associated with it.

A ribosome is a biological machine. Protein translation.gif
A ribosome is a biological machine.

The most important objectives that are frequently found in nanobiology involve applying nanotools to relevant medical/biological problems and refining these applications. Developing new tools, such as peptoid nanosheets, for medical and biological purposes is another primary objective in nanotechnology. New nanotools are often made by refining the applications of the nanotools that are already being used. The imaging of native biomolecules, biological membranes, and tissues is also a major topic for nanobiology researchers. Other topics concerning nanobiology include the use of cantilever array sensors and the application of nanophotonics for manipulating molecular processes in living cells. [3]

Recently, the use of microorganisms to synthesize functional nanoparticles has been of great interest. Microorganisms can change the oxidation state of metals.[ citation needed ] These microbial processes have opened up new opportunities for us to explore novel applications, for example, the biosynthesis of metal nanomaterials. In contrast to chemical and physical methods, microbial processes for synthesizing nanomaterials can be achieved in aqueous phase under gentle and environmentally benign conditions. This approach has become an attractive focus in current green bionanotechnology research towards sustainable development. [4]

Terminology

The terms are often used interchangeably. When a distinction is intended, though, it is based on whether the focus is on applying biological ideas or on studying biology with nanotechnology. Bionanotechnology generally refers to the study of how the goals of nanotechnology can be guided by studying how biological "machines" work and adapting these biological motifs into improving existing nanotechnologies or creating new ones. [5] [6] Nanobiotechnology, on the other hand, refers to the ways that nanotechnology is used to create devices to study biological systems. [7]

In other words, nanobiotechnology is essentially miniaturized biotechnology, whereas bionanotechnology is a specific application of nanotechnology. For example, DNA nanotechnology or cellular engineering would be classified as bionanotechnology because they involve working with biomolecules on the nanoscale. Conversely, many new medical technologies involving nanoparticles as delivery systems or as sensors would be examples of nanobiotechnology since they involve using nanotechnology to advance the goals of biology.

The definitions enumerated above will be utilized whenever a distinction between nanobio and bionano is made in this article. However, given the overlapping usage of the terms in modern parlance, individual technologies may need to be evaluated to determine which term is more fitting. As such, they are best discussed in parallel.

Concepts

Kinesin walking on a microtubule. It is a molecular biological machine that uses protein domain dynamics on nanoscales Kinesin walking.gif
Kinesin walking on a microtubule. It is a molecular biological machine that uses protein domain dynamics on nanoscales

Most of the scientific concepts in bionanotechnology are derived from other fields. Biochemical principles that are used to understand the material properties of biological systems are central in bionanotechnology because those same principles are to be used to create new technologies. Material properties and applications studied in bionanoscience include mechanical properties (e.g. deformation, adhesion, failure), electrical/electronic (e.g. electromechanical stimulation, capacitors, energy storage/batteries), optical (e.g. absorption, luminescence, photochemistry), thermal (e.g. thermomutability, thermal management), biological (e.g. how cells interact with nanomaterials, molecular flaws/defects, biosensing, biological mechanisms such as mechanosensation), nanoscience of disease (e.g. genetic disease, cancer, organ/tissue failure), as well as biological computing (e.g. DNA computing) and agriculture (target delivery of pesticides, hormones and fertilizers. [8] [9] [10] [11] The impact of bionanoscience, achieved through structural and mechanistic analyses of biological processes at nanoscale, is their translation into synthetic and technological applications through nanotechnology.

Nanobiotechnology takes most of its fundamentals from nanotechnology.[ clarification needed ] Most of the devices designed for nano-biotechnological use are directly based on other existing nanotechnologies.[ citation needed ] Nanobiotechnology is often used to describe the overlapping multidisciplinary activities associated with biosensors, particularly where photonics, chemistry, biology, biophysics, nanomedicine, and engineering converge. Measurement in biology using wave guide techniques, such as dual-polarization interferometry, is another example.

Applications

Applications of bionanotechnology are extremely widespread. Insofar as the distinction holds, nanobiotechnology is much more commonplace in that it simply provides more tools for the study of biology. Bionanotechnology, on the other hand, promises to recreate biological mechanisms and pathways in a form that is useful in other ways.

Nanomedicine

Nanomedicine is a field of medical science whose applications are increasing.

Nanobots

The field includes nanorobots and biological machines, which constitute a very useful tool to develop this area of knowledge. In the past years, researchers have made many improvements in the different devices and systems required to develop functional nanorobots – such as motion and magnetic guidance. [12] [13] This supposes a new way of treating and dealing with diseases such as cancer; thanks to nanorobots, side effects of chemotherapy could get controlled, reduced and even eliminated, so some years from now, cancer patients could be offered an alternative to treat such diseases instead of chemotherapy,[ citation needed ] which causes secondary effects such as hair loss, fatigue or nausea killing not only cancerous cells but also the healthy ones. Nanobots could be used for various therapies, surgery, diagnosis, and medical imaging [14] – such as via targeted drug-delivery to the brain (similar to nanoparticles) and other sites. [15] [16] [17] Programmability for combinations of features such as "tissue penetration, site-targeting, stimuli responsiveness, and cargo-loading" makes such nanobots promising candidates for "precision medicine". [18]

At a clinical level, cancer treatment with nanomedicine would consist of the supply of nanorobots to the patient through an injection that will search for cancerous cells while leaving the healthy ones untouched. Patients that are treated through nanomedicine would thereby not notice the presence of these nanomachines inside them; the only thing that would be noticeable is the progressive improvement of their health.[ citation needed ] Nanobiotechnology may be useful for medicine formulation.[ clarification needed ]

"Precision antibiotics" has been proposed to make use of bacteriocin-mechanisms for targeted antibiotics. [19] [20]

Nanoparticles

Nanoparticles are already widely used in medicine. Its applications overlap with those of nanobots and in some cases it may be difficult to distinguish between them. They can be used to for diagnosis and targeted drug delivery, encapsulating medicine. [21] Some can be manipulated using magnetic fields and, for example, experimentally, remote-controlled hormone release has been achieved this way. [22]

On example advanced application under development are "Trojan horse" designer-nanoparticles that makes blood cells eat away – from the inside out – portions of atherosclerotic plaque that cause heart attacks [23] [24] [25] and are the current most common cause of death globally. [26] [27]

Artificial cells

Artificial cells such as synthetic red blood cells that have all or many of the natural cells' known broad natural properties and abilities could be used to load functional cargos such as hemoglobin, drugs, magnetic nanoparticles, and ATP biosensors which may enable additional non-native functionalities. [28] [29]

Other

Nanofibers that mimic the matrix around cells and contain molecules that were engineered to wiggle was shown to be a potential therapy for spinal cord injury in mice. [30] [31] [32]

Technically, gene therapy can also be considered to be a form of nanobiotechnology or to move towards it. [33] An example of an area of genome editing related developments that is more clearly nanobiotechnology than more conventional gene therapies, is synthetic fabrication of functional materials in tissues. Researcher made C. elegans worms synthesize, fabricate, and assemble bioelectronic materials in its brain cells. They enabled modulation of membrane properties in specific neuron populations and manipulation of behavior in the living animals which might be useful in the study and treatments for diseases such as multiple sclerosis in specific and demonstrates the viability of such synthetic in vivo fabrication. [34] [35] [36] Moreover, such genetically modified neurons may enable connecting external components – such as prosthetic limbs – to nerves. [37]

Nanosensors based on e.g. nanotubes, nanowires, cantilevers, or atomic force microscopy could be applied to diagnostic devices/sensors [21]

Nanobiotechnology

Nanobiotechnology (sometimes referred to as nanobiology) in medicine may be best described as helping modern medicine progress from treating symptoms to generating cures and regenerating biological tissues.

Three American patients have received whole cultured bladders with the help of doctors who use nanobiology techniques in their practice. Also, it has been demonstrated in animal studies that a uterus can be grown outside the body and then placed in the body in order to produce a baby. Stem cell treatments have been used to fix diseases that are found in the human heart and are in clinical trials in the United States. There is also funding for research into allowing people to have new limbs without having to resort to prosthesis. Artificial proteins might also become available to manufacture without the need for harsh chemicals and expensive machines. It has even been surmised that by the year 2055, computers may be made out of biochemicals and organic salts. [38]

In vivo biosensors

Another example of current nanobiotechnological research involves nanospheres coated with fluorescent polymers. Researchers are seeking to design polymers whose fluorescence is quenched when they encounter specific molecules. Different polymers would detect different metabolites. The polymer-coated spheres could become part of new biological assays, and the technology might someday lead to particles which could be introduced into the human body to track down metabolites associated with tumors and other health problems. Another example, from a different perspective, would be evaluation and therapy at the nanoscopic level, i.e. the treatment of nanobacteria (25-200 nm sized) as is done by NanoBiotech Pharma.[ citation needed ]

In vitro biosensors

"Nanoantennas" made out of DNA – a novel type of nano-scale optical antenna – can be attached to proteins and produce a signal via fluorescence when these perform their biological functions, in particular for their distinct conformational changes. This could be used for further nanobiotechnology such as various types of nanomachines, to develop new drugs, for bioresearch and for new avenues in biochemistry. [39] [40]

Energy

It may also be useful in sustainable energy: in 2022, researchers reported 3D-printed nano-"skyscraper" electrodes – albeit micro-scale, the pillars had nano-features of porosity due to printed metal nanoparticle inks – (nanotechnology) that house cyanobacteria for extracting substantially more sustainable bioenergy from their photosynthesis (biotechnology) than in earlier studies. [41] [42] [43] [44] [45]

Nanobiology

While nanobiology is in its infancy, there are a lot of promising methods that may rely on nanobiology in the future. Biological systems are inherently nano in scale; nanoscience must merge with biology in order to deliver biomacromolecules and molecular machines that are similar to nature. Controlling and mimicking the devices and processes that are constructed from molecules is a tremendous challenge to face for the converging disciplines of nanobiotechnology. [46] All living things, including humans, can be considered to be nanofoundries. Natural evolution has optimized the "natural" form of nanobiology over millions of years. In the 21st century, humans have developed the technology to artificially tap into nanobiology. This process is best described as "organic merging with synthetic". Colonies of live neurons can live together on a biochip device; according to research from Gunther Gross at the University of North Texas. Self-assembling nanotubes have the ability to be used as a structural system. They would be composed together with rhodopsins; which would facilitate the optical computing process and help with the storage of biological materials. DNA (as the software for all living things) can be used as a structural proteomic system – a logical component for molecular computing. Ned Seeman – a researcher at New York University – along with other researchers are currently researching concepts that are similar to each other. [47]

Bionanotechnology

Distinction from nanobiotechnology

Broadly, bionanotechnology can be distinguished from nanobiotechnology in that it refers to nanotechnology that makes use of biological materials/components – it could in principle or does alternatively use abiotic components. It plays a smaller role in medicine (which is concerned with biological organisms). It makes use of natural or biomimetic systems or elements for unique nanoscale structures and various applications that may not be directionally associated with biology rather than mostly biological applications. In contrast, nanobiotechnology uses biotechnology miniaturized to nanometer size or incorporates nanomolecules into biological systems. In some future applications, both fields could be merged. [48] [49] [50] [ additional citation(s) needed ]

DNA

DNA nanotechnology is one important example of bionanotechnology. [51] The utilization of the inherent properties of nucleic acids like DNA to create useful materials or devices – such as biosensors [52] – is a promising area of modern research.

DNA digital data storage refers mostly to the use of synthesized but otherwise conventional strands of DNA to store digital data, which could be useful for e.g. high-density long-term data storage [53] that isn't accessed and written to frequently as an alternative to 5D optical data storage or for use in combination with other nanobiotechnology.

Membrane materials

Another important area of research involves taking advantage of membrane properties to generate synthetic membranes. Proteins that self-assemble to generate functional materials could be used as a novel approach for the large-scale production of programmable nanomaterials. One example is the development of amyloids found in bacterial biofilms as engineered nanomaterials that can be programmed genetically to have different properties. [54]

Lipid nanotechnology

Lipid nanotechnology is another major area of research in bionanotechnology, where physico-chemical properties of lipids such as their antifouling and self-assembly is exploited to build nanodevices with applications in medicine and engineering. [55] Lipid nanotechnology approaches can also be used to develop next-generation emulsion methods to maximize both absorption of fat-soluble nutrients and the ability to incorporate them into popular beverages. [56]

Computing

"Memristors" fabricated from protein nanowires of the bacterium Geobacter sulfurreducens which function at substantially lower voltages than previously described ones may allow the construction of artificial neurons which function at voltages of biological action potentials. The nanowires have a range of advantages over silicon nanowires and the memristors may be used to directly process biosensing signals, for neuromorphic computing (see also: wetware computer) and/or direct communication with biological neurons. [57] [58] [59]

Other

Protein folding studies provide a third important avenue of research, but one that has been largely inhibited by our inability to predict protein folding with a sufficiently high degree of accuracy. Given the myriad uses that biological systems have for proteins, though, research into understanding protein folding is of high importance and could prove fruitful for bionanotechnology in the future.[ citation needed ]

Agriculture

In the agriculture industry, engineered nanoparticles have been serving as nano carriers, containing herbicides, chemicals, or genes, which target particular plant parts to release their content. [60] [61]

Previously nanocapsules containing herbicides have been reported to effectively penetrate through cuticles and tissues, allowing the slow and constant release of the active substances. Likewise, other literature describes that nano-encapsulated slow release of fertilizers has also become a trend to save fertilizer consumption and to minimize environmental pollution through precision farming. These are only a few examples from numerous research works which might open up exciting opportunities for nanobiotechnology application in agriculture. Also, application of this kind of engineered nanoparticles to plants should be considered the level of amicability before it is employed in agriculture practices. Based on a thorough literature survey, it was understood that there is only limited authentic information available to explain the biological consequence of engineered nanoparticles on treated plants. Certain reports underline the phytotoxicity of various origin of engineered nanoparticles to the plant caused by the subject of concentrations and sizes . At the same time, however, an equal number of studies were reported with a positive outcome of nanoparticles, which facilitate growth promoting nature to treat plant. [62] In particular, compared to other nanoparticles, silver and gold nanoparticles based applications elicited beneficial results on various plant species with less and/or no toxicity. [63] [64] Silver nanoparticles (AgNPs) treated leaves of Asparagus showed the increased content of ascorbate and chlorophyll. Similarly, AgNPs-treated common bean and corn has increased shoot and root length, leaf surface area, chlorophyll, carbohydrate and protein contents reported earlier. [65] The gold nanoparticle has been used to induce growth and seed yield in Brassica juncea. [66]

Tools

This field relies on a variety of research methods, including experimental tools (e.g. imaging, characterization via AFM/optical tweezers etc.), x-ray diffraction based tools, synthesis via self-assembly, characterization of self-assembly (using e.g. MP-SPR, DPI, recombinant DNA methods, etc.), theory (e.g. statistical mechanics, nanomechanics, etc.), as well as computational approaches (bottom-up multi-scale simulation, supercomputing).

Risk management

As of 2009, the risks of nanobiotechnologies are poorly understood and in the U.S. there is no solid national consensus on what kind of regulatory policy principles should be followed. [33] For example, nanobiotechnologies may have hard to control effects on the environment or ecosystems and human health. The metal-based nanoparticles used for biomedical prospectives are extremely enticing in various applications due to their distinctive physicochemical characteristics, allowing them to influence cellular processes at the biological level. The fact that metal-based nanoparticles have high surface-to-volume ratios makes them reactive or catalytic. Due to their small size, they are more likely to be able to penetrate biological barriers such as cell membranes and cause cellular dysfunction in living organisms. Indeed, the high toxicity of some transition metals can make it challenging to use mixed oxide NPs in biomedical uses. It triggers adverse effects on organisms, causing oxidative stress, stimulating the formation of ROS, mitochondrial perturbation, and the modulation of cellular functions, with fatal results in some cases. [67]

Bonin notes that "Nanotechnology is not a specific determinate homogenous entity, but a collection of diverse capabilities and applications" and that nanobiotechnology research and development is – as one of many fields – affected by dual-use problems. [68]

See also

Related Research Articles

<span class="mw-page-title-main">Nanotechnology</span> Field of science involving control of matter on atomic and (supra)molecular scales

Nanotechnology was defined by the National Nanotechnology Initiative as the manipulation of matter with at least one dimension sized from 1 to 100 nanometers (nm). At this scale, commonly known as the nanoscale, surface area and quantum mechanical effects become important in describing properties of matter. The definition of nanotechnology is inclusive of all types of research and technologies that deal with these special properties. It is therefore common to see the plural form "nanotechnologies" as well as "nanoscale technologies" to refer to the broad range of research and applications whose common trait is size. An earlier description of nanotechnology referred to the particular technological goal of precisely manipulating atoms and molecules for fabrication of macroscale products, also now referred to as molecular nanotechnology.

Nanomedicine is the medical application of nanotechnology. Nanomedicine ranges from the medical applications of nanomaterials and biological devices, to nanoelectronic biosensors, and even possible future applications of molecular nanotechnology such as biological machines. Current problems for nanomedicine involve understanding the issues related to toxicity and environmental impact of nanoscale materials.

<span class="mw-page-title-main">Molecular engineering</span> Field of study in molecular properties

Molecular engineering is an emerging field of study concerned with the design and testing of molecular properties, behavior and interactions in order to assemble better materials, systems, and processes for specific functions. This approach, in which observable properties of a macroscopic system are influenced by direct alteration of a molecular structure, falls into the broader category of “bottom-up” design.

Nanosensors are nanoscale devices that measure physical quantities and convert these to signals that can be detected and analyzed. There are several ways proposed today to make nanosensors; these include top-down lithography, bottom-up assembly, and molecular self-assembly. There are different types of nanosensors in the market and in development for various applications, most notably in defense, environmental, and healthcare industries. These sensors share the same basic workflow: a selective binding of an analyte, signal generation from the interaction of the nanosensor with the bio-element, and processing of the signal into useful metrics.

<span class="mw-page-title-main">Nanorobotics</span> Emerging technology field

Nanoid robotics, or for short, nanorobotics or nanobotics, is an emerging technology field creating machines or robots, which are called nanorobots or simply nanobots, whose components are at or near the scale of a nanometer. More specifically, nanorobotics refers to the nanotechnology engineering discipline of designing and building nanorobots with devices ranging in size from 0.1 to 10 micrometres and constructed of nanoscale or molecular components. The terms nanobot, nanoid, nanite, nanomachine and nanomite have also been used to describe such devices currently under research and development.

<span class="mw-page-title-main">Nanoparticle</span> Particle with size less than 100 nm

A nanoparticle or ultrafine particle is a particle of matter 1 to 100 nanometres (nm) in diameter. The term is sometimes used for larger particles, up to 500 nm, or fibers and tubes that are less than 100 nm in only two directions. At the lowest range, metal particles smaller than 1 nm are usually called atom clusters instead.

<span class="mw-page-title-main">DNA origami</span> Folding of DNA to create two- and three-dimensional shapes at the nanoscale

DNA origami is the nanoscale folding of DNA to create arbitrary two- and three-dimensional shapes at the nanoscale. The specificity of the interactions between complementary base pairs make DNA a useful construction material, through design of its base sequences. DNA is a well-understood material that is suitable for creating scaffolds that hold other molecules in place or to create structures all on its own.

<span class="mw-page-title-main">Drug delivery</span> Methods for delivering drugs to target sites

Drug delivery refers to approaches, formulations, manufacturing techniques, storage systems, and technologies involved in transporting a pharmaceutical compound to its target site to achieve a desired therapeutic effect. Principles related to drug preparation, route of administration, site-specific targeting, metabolism, and toxicity are used to optimize efficacy and safety, and to improve patient convenience and compliance. Drug delivery is aimed at altering a drug's pharmacokinetics and specificity by formulating it with different excipients, drug carriers, and medical devices. There is additional emphasis on increasing the bioavailability and duration of action of a drug to improve therapeutic outcomes. Some research has also been focused on improving safety for the person administering the medication. For example, several types of microneedle patches have been developed for administering vaccines and other medications to reduce the risk of needlestick injury.

<span class="mw-page-title-main">Molecular biophysics</span> Interdisciplinary research area

Molecular biophysics is a rapidly evolving interdisciplinary area of research that combines concepts in physics, chemistry, engineering, mathematics and biology. It seeks to understand biomolecular systems and explain biological function in terms of molecular structure, structural organization, and dynamic behaviour at various levels of complexity. This discipline covers topics such as the measurement of molecular forces, molecular associations, allosteric interactions, Brownian motion, and cable theory. Additional areas of study can be found on Outline of Biophysics. The discipline has required development of specialized equipment and procedures capable of imaging and manipulating minute living structures, as well as novel experimental approaches.

Nanotoxicology is the study of the toxicity of nanomaterials. Because of quantum size effects and large surface area to volume ratio, nanomaterials have unique properties compared with their larger counterparts that affect their toxicity. Of the possible hazards, inhalation exposure appears to present the most concern, with animal studies showing pulmonary effects such as inflammation, fibrosis, and carcinogenicity for some nanomaterials. Skin contact and ingestion exposure are also a concern.

Biological computers use biologically derived molecules — such as DNA and/or proteins — to perform digital or real computations.

<span class="mw-page-title-main">Bacillus virus phi29</span> Species of virus

Bacillus virus Φ29 is a double-stranded DNA (dsDNA) bacteriophage with a prolate icosahedral head and a short tail that belongs to the genus Salasvirus, order Caudovirales, and family Salasmaviridae. They are in the same order as phages PZA, Φ15, BS32, B103, M2Y (M2), Nf, and GA-1. First discovered in 1965, the Φ29 phage is the smallest Bacillus phage isolated to date and is among the smallest known dsDNA phages.

The following outline is provided as an overview of and topical guide to nanotechnology:

<span class="mw-page-title-main">DNA nanotechnology</span> The design and manufacture of artificial nucleic acid structures for technological uses

DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, and functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in X-ray crystallography and nuclear magnetic resonance spectroscopy of proteins to determine structures. Potential applications in molecular scale electronics and nanomedicine are also being investigated.

<span class="mw-page-title-main">Nanoparticle–biomolecule conjugate</span> Tailored macromolecule with covalently-bonded bio-active substances targeting specific tissues

A nanoparticle–biomolecule conjugate is a nanoparticle with biomolecules attached to its surface. Nanoparticles are minuscule particles, typically measured in nanometers (nm), that are used in nanobiotechnology to explore the functions of biomolecules. Properties of the ultrafine particles are characterized by the components on their surfaces more so than larger structures, such as cells, due to large surface area-to-volume ratios. Large surface area-to-volume-ratios of nanoparticles optimize the potential for interactions with biomolecules.

<span class="mw-page-title-main">Spherical nucleic acid</span>

Spherical nucleic acids (SNAs) are nanostructures that consist of a densely packed, highly oriented arrangement of linear nucleic acids in a three-dimensional, spherical geometry. This novel three-dimensional architecture is responsible for many of the SNA's novel chemical, biological, and physical properties that make it useful in biomedicine and materials synthesis. SNAs were first introduced in 1996 by Chad Mirkin’s group at Northwestern University.

The applications of nanotechnology, commonly incorporate industrial, medicinal, and energy uses. These include more durable construction materials, therapeutic drug delivery, and higher density hydrogen fuel cells that are environmentally friendly. Being that nanoparticles and nanodevices are highly versatile through modification of their physiochemical properties, they have found uses in nanoscale electronics, cancer treatments, vaccines, hydrogen fuel cells, and nanographene batteries.

Magnetogenetics is a medical research technique whereby magnetic fields are used to affect cell function.

Virus nanotechnology is the use of viruses as a source of nanoparticles for biomedical purposes. Viruses are made up of a genome and a capsid; and some viruses are enveloped. Most virus capsids measure between 20-500 nm in diameter. Because of their nanometer size dimensions, viruses have been considered as naturally occurring nanoparticles. Virus nanoparticles have been subject to the nanoscience and nanoengineering disciplines. Viruses can be regarded as prefabricated nanoparticles. Many different viruses have been studied for various applications in nanotechnology: for example, mammalian viruses are being developed as vectors for gene delivery, and bacteriophages and plant viruses have been used in drug delivery and imaging applications as well as in vaccines and immunotherapy intervention.

<span class="mw-page-title-main">Intracellular delivery</span> Scientific research area

Intracellular delivery is the process of introducing external materials into living cells. Materials that are delivered into cells include nucleic acids, proteins, peptides, impermeable small molecules, synthetic nanomaterials, organelles, and micron-scale tracers, devices and objects. Such molecules and materials can be used to investigate cellular behavior, engineer cell operations or correct a pathological function.

References

  1. ↑ Ehud Gazit, Plenty of room for biology at the bottom: An introduction to bionanotechnology. Imperial College Press, 2007, ISBN   978-1-86094-677-6
  2. ↑ "Nanobiology". Nanotech-Now.com.
  3. ↑ "Nanobiology". Swiss Nanoscience Institute.
  4. ↑ Ng, CK; Sivakumar K; Liu X; Madhaiyan M; Ji L; Yang L; Tang C; Song H; Kjelleberg S; Cao B. (4 Feb 2013). "Influence of outer membrane c-type cytochromes on particle size and activity of extracellular nanoparticles produced by Shewanella oneidensis". Biotechnology and Bioengineering. 110 (7): 1831–7. doi:10.1002/bit.24856. PMID   23381725. S2CID   5903382.
  5. ↑ Bionanotechnology - Definition, wordiQ.com
  6. ↑ Nolting B, “Biophysical Nanotechnology”. In: “Methods in Modern Biophysics”, Springer, 2005, ISBN   3-540-27703-X
  7. ↑ NBTC Homepage | Nanobiotechnology Center
  8. ↑ GarciaAnoveros, J; Corey, DP (1997). "The molecules of mechanosensation". Annual Review of Neuroscience. 20: 567–94. doi:10.1146/annurev.neuro.20.1.567. PMID   9056725.
  9. ↑ Callaway DJ, Matsui T, Weiss T, Stingaciu LR, Stanley CB, Heller WT, Bu ZM (7 April 2017). "Controllable Activation of Nanoscale Dynamics in a Disordered Protein Alters Binding Kinetics". Journal of Molecular Biology. 427 (7): 987–998. doi:10.1016/j.jmb.2017.03.003. PMC   5399307 . PMID   28285124.
  10. ↑ Langer, Robert (2010). "Nanotechnology in Drug Delivery and Tissue Engineering: From Discovery to Applications". Nano Lett. 10 (9): 3223–30. Bibcode:2010NanoL..10.3223S. doi:10.1021/nl102184c. PMC   2935937 . PMID   20726522.
  11. ↑ Thangavelu, Raja Muthuramalingam; Gunasekaran, Dharanivasan; Jesse, Michael Immanuel; s.u, Mohammed Riyaz; Sundarajan, Deepan; Krishnan, Kathiravan (2018). "Nanobiotechnology approach using plant rooting hormone synthesized silver nanoparticle as "nanobullets" for the dynamic applications in horticulture – an in vitro and ex vitro study". Arabian Journal of Chemistry. 11: 48–61. doi: 10.1016/j.arabjc.2016.09.022 .
  12. ↑ Wavhale, Ravindra D.; Dhobale, Kshama D.; Rahane, Chinmay S.; Chate, Govind P.; Tawade, Bhausaheb V.; Patil, Yuvraj N.; Gawade, Sandesh S.; Banerjee, Shashwat S. (18 November 2021). "Water-powered self-propelled magnetic nanobot for rapid and highly efficient capture of circulating tumor cells". Communications Chemistry. 4 (1): 159. doi: 10.1038/s42004-021-00598-9 . ISSN   2399-3669. PMC   9814645 . PMID   36697678. S2CID   244274928.
  13. ↑ Arvidsson, Rickard; Foss Hansen, Steffen (2020). "Environmental and health risks of nanorobots: an early review". Environmental Science: Nano. 7 (10): 2875–2886. doi: 10.1039/D0EN00570C . S2CID   225154263.
  14. ↑ Soto, Fernando; Wang, Jie; Ahmed, Rajib; Demirci, Utkan (2020). "Medical Micro/Nanorobots in Precision Medicine". Advanced Science. 7 (21): 2002203. doi:10.1002/advs.202002203. ISSN   2198-3844. PMC   7610261 . PMID   33173743.
  15. ↑ Mair, Lamar O.; Adam, Georges; Chowdhury, Sagar; Davis, Aaron; Arifin, Dian R.; Vassoler, Fair M.; Engelhard, Herbert H.; Li, Jinxing; Tang, Xinyao; Weinberg, Irving N.; Evans, Benjamin A.; Bulte, Jeff W.M.; Cappelleri, David J. (2021). "Soft Capsule Magnetic Millirobots for Region-Specific Drug Delivery in the Central Nervous System". Frontiers in Robotics and AI. 8: 702566. doi: 10.3389/frobt.2021.702566 . ISSN   2296-9144. PMC   8340882 . PMID   34368238.
  16. ↑ Zhang, Hongyue; Li, Zesheng; Gao, Changyong; Fan, Xinjian; Pang, Yuxin; Li, Tianlong; Wu, Zhiguang; Xie, Hui; He, Qiang (24 March 2021). "Dual-responsive biohybrid neutrobots for active target delivery". Science Robotics. 6 (52). doi:10.1126/scirobotics.aaz9519. PMID   34043546. S2CID   232368379.
  17. ↑ Rojas, Carlos de (20 October 2021). "Arming Biological Nanobots to Deliver Drugs Inside Our Bodies". Labiotech.eu. Retrieved 30 January 2022.
  18. ↑ Hu, Yong (19 October 2021). "Self-Assembly of DNA Molecules: Towards DNA Nanorobots for Biomedical Applications". Cyborg and Bionic Systems. 2021: 1–3. doi:10.34133/2021/9807520. PMC   9494698 . PMID   36285141. S2CID   239462084.
  19. ↑ "Bactericidal nanomachine: Researchers reveal the mechanisms behind a natural bacteria killer". phys.org. Retrieved 17 May 2020.
  20. ↑ Ge, Peng; Scholl, Dean; Prokhorov, Nikolai S.; Avaylon, Jaycob; Shneider, Mikhail M.; Browning, Christopher; Buth, Sergey A.; Plattner, Michel; Chakraborty, Urmi; Ding, Ke; Leiman, Petr G.; Miller, Jeff F.; Zhou, Z. Hong (April 2020). "Action of a minimal contractile bactericidal nanomachine". Nature. 580 (7805): 658–662. Bibcode:2020Natur.580..658G. doi:10.1038/s41586-020-2186-z. PMC   7513463 . PMID   32350467. S2CID   215774771.
  21. 1 2 Nasimi, Parva; Haidari, Maryam (1 January 2013). "Medical Use of Nanoparticles". International Journal of Green Nanotechnology. 1: 194308921350697. doi:10.1177/1943089213506978. ISSN   1943-0906.
  22. ↑ Rosenfeld, Dekel; Senko, Alexander W.; Moon, Junsang; Yick, Isabel; Varnavides, Georgios; Gregureć, Danijela; Koehler, Florian; Chiang, Po-Han; Christiansen, Michael G.; Maeng, Lisa Y.; Widge, Alik S.; Anikeeva, Polina (April 2020). "Transgene-free remote magnetothermal regulation of adrenal hormones". Science Advances. 6 (15): eaaz3734. Bibcode:2020SciA....6.3734R. doi:10.1126/sciadv.aaz3734. PMC   7148104 . PMID   32300655.
  23. ↑ "Nanoparticle chomps away plaques that cause heart attacks". Michigan State University. 27 January 2020. Retrieved 31 January 2020.
  24. ↑ "Nanoparticle helps eat away deadly arterial plaque". New Atlas. 28 January 2020. Retrieved 13 April 2020.
  25. ↑ Flores, Alyssa M.; Hosseini-Nassab, Niloufar; Jarr, Kai-Uwe; Ye, Jianqin; Zhu, Xingjun; Wirka, Robert; Koh, Ai Leen; Tsantilas, Pavlos; Wang, Ying; Nanda, Vivek; Kojima, Yoko; Zeng, Yitian; Lotfi, Mozhgan; Sinclair, Robert; Weissman, Irving L.; Ingelsson, Erik; Smith, Bryan Ronain; Leeper, Nicholas J. (February 2020). "Pro-efferocytic nanoparticles are specifically taken up by lesional macrophages and prevent atherosclerosis". Nature Nanotechnology. 15 (2): 154–161. Bibcode:2020NatNa..15..154F. doi:10.1038/s41565-019-0619-3. PMC   7254969 . PMID   31988506.
  26. ↑ "Fundamental beliefs about atherosclerosis overturned: Complications of artery-hardening condition are number one killer worldwide". ScienceDaily.
  27. ↑ "The top 10 causes of death". www.who.int. Retrieved 2020-01-26.
  28. ↑ "Synthetic red blood cells mimic natural ones, and have new abilities". phys.org. Retrieved 13 June 2020.
  29. ↑ Guo, Jimin; Agola, Jacob Ongudi; Serda, Rita; Franco, Stefan; Lei, Qi; Wang, Lu; Minster, Joshua; Croissant, Jonas G.; Butler, Kimberly S.; Zhu, Wei; Brinker, C. Jeffrey (11 May 2020). "Biomimetic Rebuilding of Multifunctional Red Blood Cells: Modular Design Using Functional Components". ACS Nano. 14 (7): 7847–7859. doi:10.1021/acsnano.9b08714. OSTI   1639054. PMID   32391687. S2CID   218584795.
  30. ↑ "Therapy used on mice may transform spinal injury treatments, say scientists". The Guardian. 11 November 2021. Retrieved 11 December 2021.
  31. ↑ University. "'Dancing molecules' successfully repair severe spinal cord injuries in mice". Northwestern University . Retrieved 11 December 2021.
  32. ↑ Álvarez, Z.; Kolberg-Edelbrock, A. N.; Sasselli, I. R.; Ortega, J. A.; Qiu, R.; Syrgiannis, Z.; Mirau, P. A.; Chen, F.; Chin, S. M.; Weigand, S.; Kiskinis, E.; Stupp, S. I. (12 November 2021). "Bioactive scaffolds with enhanced supramolecular motion promote recovery from spinal cord injury". Science. 374 (6569): 848–856. Bibcode:2021Sci...374..848A. doi:10.1126/science.abh3602. PMC   8723833 . PMID   34762454. S2CID   244039388.
  33. 1 2 Hornig Priest, Susanna. "Risk Communication for Nanobiotechnology: To Whom, About What, and Why?" (PDF). Archived from the original (PDF) on 22 October 2020.
  34. ↑ "Scientists program cells to carry out gene-guided construction projects". phys.org. Retrieved 5 April 2020.
  35. ↑ Otto, Kevin J.; Schmidt, Christine E. (20 March 2020). "Neuron-targeted electrical modulation". Science. 367 (6484): 1303–1304. Bibcode:2020Sci...367.1303O. doi:10.1126/science.abb0216. PMID   32193309. S2CID   213192749.
  36. ↑ Liu, Jia; Kim, Yoon Seok; Richardson, Claire E.; Tom, Ariane; Ramakrishnan, Charu; Birey, Fikri; Katsumata, Toru; Chen, Shucheng; Wang, Cheng; Wang, Xiao; Joubert, Lydia-Marie; Jiang, Yuanwen; Wang, Huiliang; Fenno, Lief E.; Tok, Jeffrey B.-H.; Pașca, Sergiu P.; Shen, Kang; Bao, Zhenan; Deisseroth, Karl (20 March 2020). "Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals". Science. 367 (6484): 1372–1376. Bibcode:2020Sci...367.1372L. doi:10.1126/science.aay4866. PMC   7527276 . PMID   32193327. S2CID   213191980.
  37. ↑ "Genetically modified neurons could help us connect to implants". New Scientist. Retrieved 1 February 2022.
  38. ↑ "The future of nano-biology". ZD Net.
  39. ↑ "Chemists use DNA to build the world's tiniest antenna". University of Montreal. Retrieved 19 January 2022.
  40. ↑ Harroun, Scott G.; Lauzon, Dominic; Ebert, Maximilian C. C. J. C.; Desrosiers, Arnaud; Wang, Xiaomeng; Vallée-Bélisle, Alexis (January 2022). "Monitoring protein conformational changes using fluorescent nanoantennas". Nature Methods. 19 (1): 71–80. doi: 10.1038/s41592-021-01355-5 . ISSN   1548-7105. PMID   34969985. S2CID   245593311.
  41. ↑ "Tiny 'skyscrapers' help bacteria convert sunlight into electricity". University of Cambridge . Retrieved 19 April 2022.
  42. ↑ Franco, Louise (24 March 2022). "This Bacteria Can Consume Greenhouse Gas Methane and Convert it Into Fuel" . Retrieved 28 April 2022.
  43. ↑ "Tiny Skyscrapers help generate more electricity from cyanobacteria". BioTechniques. 15 March 2022. Retrieved 28 April 2022.
  44. ↑ ""Tiny skyscraper" electrodes boost bioenergy output of blue-green algae". New Atlas. 8 March 2022. Retrieved 28 April 2022.
  45. ↑ Chen, Xiaolong; Lawrence, Joshua M.; Wey, Laura T.; Schertel, Lukas; Jing, Qingshen; Vignolini, Silvia; Howe, Christopher J.; Kar-Narayan, Sohini; Zhang, Jenny Z. (7 March 2022). "3D-printed hierarchical pillar array electrodes for high-performance semi-artificial photosynthesis". Nature Materials. 21 (7): 811–818. doi:10.1038/s41563-022-01205-5. ISSN   1476-4660. PMID   35256790. S2CID   247255146.
  46. ↑ Nussinov, Ruth; Alemán, Carlos (2006). "Nanobiology: from physics and engineering to biology". Physical Biology. 3. IOP Science. doi: 10.1088/1478-3975/3/1/E01 .
  47. ↑ "The Nanobiology Imperative". HistorianoftheFuture.com.
  48. ↑ "Introduction: Nanobiotechnology and Bionanotechnology". Plenty of Room for Biology at the Bottom. Imperial College Press. 1 February 2007. pp. 1–15. doi:10.1142/9781860948190_0001. ISBN   978-1-86094-677-6.
  49. ↑ Petrovykh, Dmitri. "Biointerface: Nanobiotechnology and Bionanotechnology". biointerface.org. Retrieved 24 April 2022.
  50. ↑ Wei, Shuaifei (21 May 2018). "Nanotechnology and Biotechnology - Similarities and Differences". AZoNano.com. Retrieved 28 April 2022.
  51. ↑ Zadegan, Reza M.; Norton, Michael L. (June 2012). "Structural DNA Nanotechnology: From Design to Applications". Int. J. Mol. Sci. 13 (6): 7149–7162. doi: 10.3390/ijms13067149 . PMC   3397516 . PMID   22837684.
  52. ↑ Jung, Jaeyoung K.; Archuleta, Chloé M.; Alam, Khalid K.; Lucks, Julius B. (17 February 2022). "Programming cell-free biosensors with DNA strand displacement circuits". Nature Chemical Biology. 18 (4): 385–393. doi:10.1038/s41589-021-00962-9. ISSN   1552-4469. PMC   8964419 . PMID   35177837. S2CID   246901702.
  53. ↑ "Scientists claim big advance in using DNA to store data". bbc.co.uk. 2 December 2021. Retrieved 3 December 2021.
  54. ↑ Nguyen, Peter; Botyanszki, Zsofia; Tay, Pei-Kun; Joshi, Neel (Sep 17, 2014). "Programmable biofilm-based materials from engineered curli nanofibres" (PDF). Nature Communications. 5: 4945. Bibcode:2014NatCo...5.4945N. doi: 10.1038/ncomms5945 . PMID   25229329.
  55. ↑ Mashaghi S.; Jadidi T.; Koenderink G.; Mashaghi A. (2013). "Lipid Nanotechnology". Int. J. Mol. Sci. 14 (2): 4242–4282. doi: 10.3390/ijms14024242 . PMC   3588097 . PMID   23429269.
  56. ↑ using-nanotechnology-to-create-beverages-infused-with-cbd-and-omega-3-fatty-acids, axiomm.com - 2020
  57. ↑ "Scientists create tiny devices that work like the human brain" . The Independent. 20 April 2020. Archived from the original on 2022-06-18. Retrieved 17 May 2020.
  58. ↑ "Researchers unveil electronics that mimic the human brain in efficient learning". phys.org. Retrieved 17 May 2020.
  59. ↑ Fu, Tianda; Liu, Xiaomeng; Gao, Hongyan; Ward, Joy E.; Liu, Xiaorong; Yin, Bing; Wang, Zhongrui; Zhuo, Ye; Walker, David J. F.; Joshua Yang, J.; Chen, Jianhan; Lovley, Derek R.; Yao, Jun (20 April 2020). "Bioinspired bio-voltage memristors". Nature Communications. 11 (1): 1861. Bibcode:2020NatCo..11.1861F. doi: 10.1038/s41467-020-15759-y . PMC   7171104 . PMID   32313096.
  60. ↑ Raja; et al. (2016). "Nanobiotechnological approach using plant rooting hormones synthesized silver nanoparticle as a nanobullets for the dynamic applications in horticulture -An in vitro and ex vitro study". Arabian Journal of Chemistry. 11: 48–61. doi: 10.1016/j.arabjc.2016.09.022 .
  61. ↑ thangavelu, Raja muthuramalingam (2019). "Effect Of Deoxycholate Capped Silver nanoparticles In Seed Dormancy Breaking Of Withania Somnifera" (PDF). Current Science. 116 (6): 952. doi: 10.18520/cs/v116/i6/952-958 .
  62. ↑ Raja; et al. (2016). "Nanobiotechnological approach using plant rooting hormones synthesized silver nanoparticle as a "nanobullets" for the dynamic applications in horticulture -An in vitro and ex vitro study". Arabian Journal of Chemistry. 11: 48–61. doi: 10.1016/j.arabjc.2016.09.022 .
  63. ↑ Raja; Chandrasekar, S.; Dharanivasan, G.; Nallusamy, D.; Rajendran, N.; Kathiravan, K. (2015). "Bioactive bile salt capped silver nanoparticle activity against destructive plant pathogenic fungi through in vitro system". RSC Advances. 5 (87): 71174–71182. Bibcode:2015RSCAd...571174R. doi:10.1039/c5ra13306h.
  64. ↑ Raqual, B.; Eudald, C.; Joan, C.; Xavier, F.; Antoni, S.; Victor, P. (2009). "Evaluation of the ecotoxicity of model nanoparticles". Chemosphere. 75 (7): 850–857. Bibcode:2009Chmsp..75..850B. doi:10.1016/j.chemosphere.2009.01.078. PMID   19264345.
  65. ↑ Hediat Salama, M. H. (2012). "Effects of silver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.)". International Research Journal of Biotechnology. 3 (10): 190–197.
  66. ↑ Arora, Sandeep; Sharma, Priyadarshini; Kumar, Sumit; Nayan, Rajeev; Khanna, P. K.; Zaidi, M. G. N. (2012). "Gold nanoparticles induced enhancement in growth and seed yield of Brassica juncea". Plant Growth Regul. 66 (3): 303–310. doi:10.1007/s10725-011-9649-z. S2CID   17018032.
  67. ↑ Min, Y.; Suminda, G.G.D.; Heo, Y.; Kim, M.; Ghosh, M.; Son, Y.-O. Metal-Based Nanoparticles and Their Relevant Consequences on Cytotoxicity Cascade and Induced Oxidative Stress. Antioxidants 2023, 12, 703. https://doi.org/10.3390/antiox12030703
  68. ↑ "Challenges to Biosecurity from Advances in the Life Sciences". United Nations. Retrieved 1 February 2022.
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