DCPS (gene)

Last updated
DCPS
Protein DCPS PDB 1st0.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases DCPS , DCS1, HINT-5, HINT5, HSL1, ARS, HSPC015, decapping enzyme, scavenger
External IDs OMIM: 610534 MGI: 1916555 HomoloGene: 32202 GeneCards: DCPS
Orthologs
SpeciesHumanMouse
Entrez

28960

69305

Ensembl

ENSG00000110063

ENSMUSG00000032040

UniProt

Q96C86

Q9DAR7

RefSeq (mRNA)

NM_014026
NM_001350236

NM_027030

RefSeq (protein)

NP_054745
NP_001337165

NP_081306

Location (UCSC) Chr 11: 126.3 – 126.35 Mb Chr 9: 35.04 – 35.09 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse
Scavenger mRNA decapping enzyme (DcpS) N-terminal
PDB 1vlr EBI.jpg
crystal structure of mrna decapping enzyme (dcps) from mus musculus at 1.83 a resolution
Identifiers
SymbolDcpS
Pfam PF05652
InterPro IPR008594
SCOP2 1st4 / SCOPe / SUPFAM
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary
Scavenger mRNA decapping enzyme C-term binding
Identifiers
SymbolDcpS_C
Pfam PF11969
Pfam clan CL0265
SCOP2 1st4 / SCOPe / SUPFAM
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

Scavenger mRNA-decapping enzyme DcpS is a protein that in humans is encoded by the DCPS gene. [5] [6] [7]

The scavenger mRNA decapping enzymes include Dcp2 and DcpS. DcpS is a scavenger pyrophosphatase that hydrolyses the residual cap structure following 3' to 5' mRNA degradation. DcpS uses cap dinucleotides or capped oligonucleotides as substrates to release m(7)GMP (N7-methyl GMP), while Dcp2 uses capped mRNA as a substrate in order to hydrolyse the cap to release m(7)GDP (N7-methyl GDP). [8] The association of DcpS with 3' to 5' exonuclease exosome components suggests that these two activities are linked and there is a coupled exonucleolytic decay-dependent decapping pathway. The family contains a histidine triad (HIT) sequence in its C-terminal domain, with three histidines separated by hydrophobic residues. [9] The central histidine within the DcpS HIT motif is critical for decapping activity and defines the HIT motif as a new mRNA decapping domain, making DcpS the first member of the HIT family of proteins with a defined biological function.

See also

Related Research Articles

In molecular biology, the five-prime cap is a specially altered nucleotide on the 5′ end of some primary transcripts such as precursor messenger RNA. This process, known as mRNA capping, is highly regulated and vital in the creation of stable and mature messenger RNA able to undergo translation during protein synthesis. Mitochondrial mRNA and chloroplastic mRNA are not capped.

<span class="mw-page-title-main">Angiogenin</span> Protein-coding gene in the species Homo sapiens

Angiogenin (ANG) also known as ribonuclease 5 is a small 123 amino acid protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessels through the process of angiogenesis. Ang hydrolyzes cellular RNA, resulting in modulated levels of protein synthesis and interacts with DNA causing a promoter-like increase in the expression of rRNA. Ang is associated with cancer and neurological disease through angiogenesis and through activating gene expression that suppresses apoptosis.

<span class="mw-page-title-main">GMP synthase</span>

Guanosine monophosphate synthetase, also known as GMPS is an enzyme that converts xanthosine monophosphate to guanosine monophosphate.

<span class="mw-page-title-main">UPF1</span> Protein-coding gene in the species Homo sapiens

Regulator of nonsense transcripts 1 is a protein that in humans is encoded by the UPF1 gene.

<span class="mw-page-title-main">TNRC6A</span> Protein-coding gene in the species Homo sapiens

Trinucleotide repeat-containing gene 6A protein is a protein that in humans is encoded by the TNRC6A gene.

<span class="mw-page-title-main">DCP2</span> Protein found in humans

mRNA-decapping enzyme 2 is a protein that in humans is encoded by the DCP2 gene.

<span class="mw-page-title-main">ITPA</span> Protein-coding gene in the species Homo sapiens

Inosine triphosphate pyrophosphatase is an enzyme that in humans is encoded by the ITPA gene, by the rdgB gene in bacteria E.coli and the HAM1 gene in yeast S. cerevisiae; the protein is also encoded by some RNA viruses of the Potyviridae family. Two transcript variants encoding two different isoforms have been found for this gene. Also, at least two other transcript variants have been identified which are probably regulatory rather than protein-coding.

<span class="mw-page-title-main">Ubiquitin carboxyl-terminal hydrolase L5</span> Protein-coding gene in the species Homo sapiens

Ubiquitin carboxyl-terminal hydrolase isozyme L5 is an enzyme that in humans is encoded by the UCHL5 gene.

<span class="mw-page-title-main">DCP1A</span> Protein found in humans

mRNA-decapping enzyme 1A is a protein that in humans is encoded by the DCP1A gene.

<span class="mw-page-title-main">PRIM2</span> Protein-coding gene in the species Homo sapiens

DNA primase large subunit is an enzyme that in humans is encoded by the PRIM2 gene.

<span class="mw-page-title-main">RNGTT</span> Protein-coding gene in the species Homo sapiens

mRNA-capping enzyme is a protein that in humans is encoded by the RNGTT gene.

<span class="mw-page-title-main">PCYOX1</span> Protein-coding gene in the species Homo sapiens

Prenylcysteine oxidase 1 is an enzyme that in humans is encoded by the PCYOX1 gene.

<span class="mw-page-title-main">TRIT1</span> Protein-coding gene in the species Homo sapiens

tRNA isopentenyltransferase, mitochondrial is an enzyme that in humans is encoded by the TRIT1 gene.

<span class="mw-page-title-main">ATP13A3</span> Protein-coding gene in the species Homo sapiens

Probable cation-transporting ATPase 13A3 is an enzyme that in humans is encoded by the ATP13A3 gene.

<span class="mw-page-title-main">EDC3</span> Protein-coding gene in the species Homo sapiens

Enhancer of mRNA-decapping protein 3 is a protein that in humans is encoded by the EDC3 gene.

<span class="mw-page-title-main">NDOR1</span> Protein-coding gene in the species Homo sapiens

NADPH-dependent diflavin oxidoreductase 1 is an enzyme that in humans is encoded by the NDOR1 gene.

<span class="mw-page-title-main">THG1L</span> Enzyme

Probable tRNA(His) guanylyltransferase is an enzyme that in humans is encoded by the THG1L gene.

<span class="mw-page-title-main">Decapping complex</span> Eukaryotic protein complex that removes the 5 cap on mRNA

The mRNA decapping complex is a protein complex in eukaryotic cells responsible for removal of the 5' cap. The active enzyme of the decapping complex is the bilobed Nudix family enzyme Dcp2, which hydrolyzes 5' cap and releases 7mGDP and a 5'-monophosphorylated mRNA. This decapped mRNA is inhibited for translation and will be degraded by exonucleases. The core decapping complex is conserved in eukaryotes. Dcp2 is activated by Decapping Protein 1 (Dcp1) and in higher eukaryotes joined by the scaffold protein VCS. Together with many other accessory proteins, the decapping complex assembles in P-bodies in the cytoplasm.

M7GpppX diphosphatase (EC 3.6.1.59, DcpS, m7GpppX pyrophosphatase, m7GpppN m7GMP phosphohydrolase) is an enzyme with systematic name m7G5'ppp5'N m7GMP phosphohydrolase. This enzyme catalyses the following chemical reaction

M7GpppN-mRNA hydrolase (EC 3.6.1.62, DCP2, NUDT16, D10 protein, D9 protein, D10 decapping enzyme, decapping enzyme) is an enzyme with systematic name m7GpppN-mRNA m7GDP phosphohydrolase. This enzyme catalyses the following chemical reaction

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000110063 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000032040 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ Liu H, Rodgers ND, Jiao X, Kiledjian M (Aug 2002). "The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases". EMBO J. 21 (17): 4699–4708. doi:10.1093/emboj/cdf448. PMC   126188 . PMID   12198172.
  6. ↑ van Dijk E, Le Hir H, Seraphin B (Oct 2003). "DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway". Proc Natl Acad Sci U S A. 100 (21): 12081–12086. Bibcode:2003PNAS..10012081V. doi: 10.1073/pnas.1635192100 . PMC   218716 . PMID   14523240.
  7. ↑ "Entrez Gene: DCPS decapping enzyme, scavenger".
  8. ↑ Liu H, Kiledjian M (February 2006). "Decapping the message: a beginning or an end". Biochem. Soc. Trans. 34 (Pt 1): 35–8. doi:10.1042/BST20060035. PMID   16246173.
  9. ↑ Han GW, Schwarzenbacher R, McMullan D, Abdubek P, Ambing E, Axelrod H, Biorac T, Canaves JM, Chiu HJ, Dai X, Deacon AM, DiDonato M, Elsliger MA, Godzik A, Grittini C, Grzechnik SK, Hale J, Hampton E, Haugen J, Hornsby M, Jaroszewski L, Klock HE, Koesema E, Kreusch A, Kuhn P, Lesley SA, McPhillips TM, Miller MD, Moy K, Nigoghossian E, Paulsen J, Quijano K, Reyes R, Spraggon G, Stevens RC, van den Bedem H, Velasquez J, Vincent J, White A, Wolf G, Xu Q, Hodgson KO, Wooley J, Wilson IA (September 2005). "Crystal structure of an Apo mRNA decapping enzyme (DcpS) from Mouse at 1.83 A resolution". Proteins. 60 (4): 797–802. doi: 10.1002/prot.20467 . PMID   16001405. S2CID   20494085.

Further reading

This article incorporates text from the public domain Pfam and InterPro: IPR008594


This page is based on this Wikipedia article
Text is available under the CC BY-SA 4.0 license; additional terms may apply.
Images, videos and audio are available under their respective licenses.