The opisthokonts are a broad group of eukaryotes, including both the animal and fungus kingdoms. The opisthokonts, previously called the "Fungi/Metazoa group", are generally recognized as a clade. Opisthokonts together with Apusomonadida and Breviata comprise the larger clade Obazoa.
Cristidiscoidea or Nucleariae is a proposed basal holomycota clade in which Fonticula and Nucleariida emerged, as sister of the fungi. Since it is close to the divergence between the main lineages of fungi and animals, the study of Cristidiscoidea can provide crucial information on the divergent lifestyles of these groups and the evolution of opisthokonts and slime mold multicellularity. The holomycota tree is following Tedersoo et al.
Telonemia is a phylum of microscopic eukaryotes commonly known as telonemids. They are unicellular free-living flagellates with a unique combination of cell structures, including a highly complex cytoskeleton unseen in other eukaryotes.
Capsaspora is a monotypic genus containing the single species Capsaspora owczarzaki. C. owczarzaki is a single-celled eukaryote that occupies a key phylogenetic position in our understanding of the origin of animal multicellularity, as one of the closest unicellular relatives to animals. It is, together with Ministeria vibrans, a member of the Filasterea clade. This amoeboid protist has been pivotal to unravel the nature of the unicellular ancestor of animals, which has been proved to be much more complex than previously thought.
Ministeria vibrans is a bacterivorous amoeba with filopodia that was originally described to be suspended by a flagellum-like stalk attached to the substrate. Molecular and experimental work later on demonstrated the stalk is indeed a flagellar apparatus.
The cryptomonads-haptophytes assemblage is a proposed but disputed monophyletic grouping of unicellular eukaryotes that are not included in the SAR supergroup. Several alternative names have been used for the group, including Hacrobia ; CCTH ; and "Eukaryomonadae".
Holozoa is a clade of organisms that includes animals and their closest single-celled relatives, but excludes fungi and all other organisms. Together they amount to more than 1.5 million species of purely heterotrophic organisms, including around 300 unicellular species. It consists of various subgroups, namely Metazoa and the protists Choanoflagellata, Filasterea, Pluriformea and Ichthyosporea. Along with fungi and some other groups, Holozoa is part of the Opisthokonta, a supergroup of eukaryotes. Choanofila was previously used as the name for a group similar in composition to Holozoa, but its usage is discouraged now because it excludes animals and is therefore paraphyletic.
The Filozoa are a monophyletic grouping within the Opisthokonta. They include animals and their nearest unicellular relatives.
Holomycota or Nucletmycea are a basal Opisthokont clade as sister of the Holozoa. It consists of the Cristidiscoidea and the kingdom Fungi. The position of nucleariids, unicellular free-living phagotrophic amoebae, as the earliest lineage of Holomycota suggests that animals and fungi independently acquired complex multicellularity from a common unicellular ancestor and that the osmotrophic lifestyle was originated later in the divergence of this eukaryotic lineage. Opisthosporidians is a recently proposed taxonomic group that includes aphelids, Microsporidia and Cryptomycota, three groups of endoparasites.
Halvaria is a taxonomic grouping of protists that includes Alveolata and Stramenopiles (Heterokonta).
Ministeria is a genus of Filasterea. The species can be found in the North Atlantic Ocean and in British waters.
Choanozoa is a clade of opisthokont eukaryotes consisting of the choanoflagellates (Choanoflagellatea) and the animals. The sister-group relationship between the choanoflagellates and animals has important implications for the origin of the animals. The clade was identified in 2015 by Graham Budd and Sören Jensen, who used the name Apoikozoa. The 2018 revision of the classification first proposed by the International Society of Protistologists in 2012 recommends the use of the name Choanozoa.
Haptista is a proposed group of protists made up of centrohelids and haptophytes. Phylogenomic studies indicate that Haptista, together with Ancoracysta twista, forms a sister clade to the SAR+Telonemia supergroup, but it may also be sister to the Cryptista (+Archaeplastida). It is thus one of the earliest diverging Diaphoretickes.
Creolimax fragrantissima is a single-celled protist that occupies a key phylogenetic position to understand the origin of animals. It was isolated from the digestive tract of some marine invertebrates, mainly from the peanut worm, collected from the Northeast Pacific.
Ichthyophonus hoferi is a single-celled protist that occupies a key phylogenetic position to understand the origin of animals. It has chitin cell wall, hyphae, and an amoeboid stage. It is a common parasite of marine and freshwater fishes.
Sphaeroforma arctica, is a unicellular eukaryote with a pivotal position in the tree of life. It was first isolated from the arctic marine amphipod Gammarus setosus. Like other Ichthyosporeans such as Creolimax and Abeoforma, Sphaeroforma arctica are spherical cells characterized with their capacity to grow into multi-nucleated coenocytes. However, a unique feature of S. arctica, is that no obvious budding, hyphal, amoeboid, sporal or flagellated growth stages have been observed in laboratory growth conditions.
Parvularia atlantis is a filopodiated amoeba which was isolated from a lake in Atlanta and deposited in the American Type Culture Collection (ATCC) under the name Nuclearia sp. ATCC 50694 on 1997 by TK Sawyer. It was classified under the genus Nuclearia and morphologically resembles to Nuclearia species, although it is smaller. Later it was determined that it phylogenetically belongs to a new nucleariid lineage., distantly related to Nuclearia and Fonticula genera – the other two previously described nucleriid genera.
Tunicaraptor is a genus of marine microbial protists containing the single species Tunicaraptor unikontum, discovered in 2020 from marine waters of Chile. It is a lineage of predatorial flagellates closely related to animals. It has a rare feeding structure not seen in other opisthokonts.
Syssomonas is a monotypic genus of unicellular flagellated protists containing the species Syssomonas multiformis. It is a member of Pluriformea inside the lineage of Holozoa, a clade containing animals and their closest protistan relatives. It lives in freshwater habitats. It has a complex life cycle that includes unicellular amoeboid and flagellated phases, as well as multicellular aggregates, depending on the growth medium and nutritional state.
An amoeboflagellate is any eukaryotic organism capable of behaving as an amoeba and as a flagellate at some point during their life cycle. Amoeboflagellates present both pseudopodia and at least one flagellum, often simultaneously.
